genechip / microarray experiments – spending on analysis
Don’t scrimp on the analysis of your genechip / microarray experimental results! Genechip / microarray experiments are now very important and useful tools in biomedical research. Genechips, while...
View Articlea cool translational bioinformatics lab
For those of you interested in bioinformatics or taking advantage of cutting-edge bioinformatics to analyze your data (e.g. microarray, proteomics), check out the Chinnaiyan lab at the University of...
View Articlescientist age and publishing–50 is the new 30?!?!!?
Here’s an interesting article suggesting that “older scientists publish more papers”: http://www.nature.com/news/2008/081029/pdf/4551161a.pdf (if the link is down, see the October 30 2008 issue of...
View Articlepredicting microRNA binding sites
Are you interested in or researching microRNA regulation of gene expression? MicroInspector (written by Vesselin Baev and Ventsislav Rusinov at the University of Plovdiv in Bulgaria) is a web-based...
View Articlesodium borate nucleic acid electrophoresis buffer
Sodium Borate Electrophoresis Buffer (SBE buffer) is an excellent alternative to traditional TBE (TRIS borate-EDTA) and TAE (TRIS acetate-EDTA) electrophoresis buffers. SBE buffer allows for higher...
View Articlewhere to buy sodium borate electrophoresis buffer
There was some interest in the sodium borate buffer DNA electrophoresis so I thought that I would look around for companies that sell the stuff. I actually had a hard time finding anyone who sells...
View Articlewestern blotting reagent recipes
All reagents for western blot are mostly purchased by labs, but if you still make your own reagents, here are some recipes I used during graduate school. These reagents are compatible with all pre-made...
View Articlegrants, scholarships, fellowships
There are now over 100 grants, scholarships, fellowships for research (or just for you!) for medical students, graduate students, postdocs, residents, clinical fellows and investigators now on my...
View ArticleDT40 cell culture media recipe
DT40s are a cell culture line of chicken lymphocyte B cells. They exhibit a high rate of targeted (and random) integration of transfected DNA constructs and are ideal for stable transfection. They...
View Articlelittle magnetic beads saved my phd
One mainstay method for cell sorting is with flow cytometry. However, this can be a time intensive procedure and depends on the availability of a flow cytometer with sorting capability and someone who...
View Articleantibody resources for experiments
Good antibodies are critical for any research involving qualitative or quantitative protein detection, isolation, localization and visualization (e.g. western blots, immunoprecipitation or...
View ArticlePhosphoPep – a database of protein phosphorylation data
PhosphoPep is a database of mass spectometry-derived phosphorylation data currently from 4 different organisms: the fly (Drosophila melanogaster), human (Homo sapiens), worm (Caenorhabditis elegans),...
View Articlet cell homeostasis – more interesting than it sounds
Look–we all know that T cells are important right? I mean, it happens when someone is infected with HIV and loses just the subset of CD4+ T cells: AIDS. There you have it–case closed. Okay, so in a...
View Articletherapeutic interventions for endoplasmic reticulum stress
The endoplasmic reticulum (ER) is a subcellular organelle that is responsible for numerous functions including calcium homeostasis, protein secretion and lipid biosynthesis. However, cell death...
View Article“super” cytotoxic t-lymphocyte clones for control of hiv infection
One of the hallmarks of HIV infection is rapid mutation and subsequent “escape” from adaptive immune responses–in particular the CD8+ cytotoxic T lymphocyte (CTL) response, which has been shown to be...
View Articleil-12 and il-23 inhibitor shows promise for severe psoriasis
Ustekinumab is a human monoclonal antibody that antagonizes signalling of the heterodimeric cytokines interleukin 12 (IL-12) and IL-23 by binding to their shared p40 subunit. Previous work has...
View Articlehow notch heals a broken heart
From the December 22nd issue of the Journal of Experimental Medicine: The Notch pathway helps a developing heart get into shape but also helps a damaged heart reshape itself and keep pumping. Weakened...
View Articlenon-parametric statistical tests
Nonparametric tests are also referred to as distribution-free tests. These tests are used when you cannot make the assumption of normality. Non-parametric tests compare medians rather than means and,...
View Articlenash equilibrium article
You may have seen the movie “A Beautiful Mind” and heard about John Nash, the Princeton mathematician who won the Nobel prize in economics for his work on mathematically describing what has now been...
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